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Custom Protein Expression
Our custom protein expression provide bespoke solutions for expressing complex, high-quality recombinant proteins. From expression system selection and optimization to scale-up and purification, our scientists develop strategies designed around your specific protein and research requirements.
Solving Custom Protein Expression Challenges
Successful custom protein expression starts with understanding how each target behaves. Our scientists develop expression strategies around the characteristics of your protein, evaluating the conditions needed to improve yield, solubility, stability, and overall protein quality. This approach helps move challenging recombinant proteins from sequence to usable material.
Optimization
Expression conditions can impact protein yield and quality. We optimize key parameters to improve expression, solubility, stability, and recovery for each target.
Expression System Selection
Different proteins require different expression strategies. We help identify the appropriate system based on protein characteristics, downstream applications, and project requirements.
Troubleshooting
Low expression, aggregation, insolubility, and poor stability can derail a project. Our scientists investigate expression challenges and adjust strategies to improve outcomes.
Flexible Scale
Protein needs can change as research progresses. Our custom protein expression can support projects from early feasibility and small-scale expression through larger-scale requirements.
Downstream Readiness
Our custom protein expression is only valuable if the resulting protein meets your research needs. We consider downstream purification, characterization, and intended use when developing the expression strategy.
Featured Case Studies / Capabilities
Protein Expression & Protein Purification
Operating as a protein production contract research organization, we provide versatile protein expression services tailored to your project’s needs. Our custom protein expression covers mammalian expression, insect expression, bacterial expression, and yeast expression techniques.
Our support as your chosen CRO extends across multiple stages of the protein production process by also providing protein purification services, encompassing sample preparation, protein extraction, optimization, purification, analysis, and quality control.
Structural Biology Services
When you choose us as your protein production CRO, we will deploy advanced techniques such as X-Ray Crystallography, NMR spectroscopy, and cryo-electron microscopy to determine the three-dimensional structures of proteins. These services will enable researchers to explore the inner workings of proteins, and drive forward your drug discovery, and include the following:
- Target Identification
- Protein Expression Optimization
- Validation of Protein Production
- Design of Therapeutic Agents
- Mechanism of Action Studies
Biophysical Assays
By examining the physical properties of proteins, biophysical assays provide an insight into the structural and functional attributes of proteins, revealing information about their stability, interactions, and behavior in certain conditions. Through our protein production CRO, researchers will gain a comprehensive understanding of protein intricacies, and our use of diverse biophysical techniques will guide decisions in production and downstream applications.
Nuclear Magnetic Resonance: Produce structurally sound proteins with our NMR studies, which contribute to the success of downstream assays and applications, through the study of structures and dynamics of proteins, including protein conformation, interactions, and mobility.
Surface Plasmon Resonance: As a CRO specializing in protein production, our SPR assay provides validation of protein-protein interactions and assesses binding affinities of biomolecular interactions, aiding in the characterization of small molecule interactions and exploration of protein behavior.
Bio-layer Interferometry Services: Through measuring interference patterns, Bio-layer Interferometry (BLI) determines binding constants, kinetics, and the thermodynamic properties of proteins. This process characterizes protein interactions, and provides data for refinement of purification approaches which ensures quality protein.
Isothermal Titration Calorimetry: Isothermal Titration Calorimetry (ITC) measures the heat released or absorbed during binding events, allowing for the quantification of binding constants and the exploration of thermodynamic details in protein interactions, it is used to analyze the complexities of protein-ligand associations while preserving the functionality and integrity of proteins.