What is SPR?
Surface Plasmon Resonance (SPR) is an optical technique that is utilised in biophysical assays. The SPR assay is used to monitor the interactions between biomolecules, by detecting changes in the refractive index that occur when molecules bind to a sensor surface. The SPR binding assay further provides valuable information regarding the binding kinetics, affinity, and binding specificity of biomolecular interactions.
Benefits of our Biacore SPR Assay service:
Label-Free Detection: SPR assays do not require modifications to the molecules of interest, therefore the native state and functionality of the molecules is preserved, ensuring reliable and physiologically relevant data.
Real-Time Analysis: The SPR assay technology allows us to track biomolecular interactions in real-time, allowing for the evaluation of kinetic data, association rates, and dissociation rates throughout the entire binding process.
High Sensitivity and Specificity: Complex biological interactions can be detected at incredibly low concentrations, ranging from picomolar to femtomolar concentrations, with high accuracy due to the sensitivity and specificity of SPR assays.
Quantitative Insights: The data-driven approach of the SPR assay yields precise quantitative data on binding kinetics, affinities, and concentrations, offering a breadth of interaction analysis, enabling a deeper understanding of molecular interactions, so you can make informed decisions.
Affinity Determination: By enabling direct measurement of binding affinities without requiring extensive assumptions or modifications, our SPR assay provides a reliable foundation for assessing molecular interactions, contributing to the precision of drug development and biomolecular research.
Applications of SPR Assays
Drug Discovery: The SPR assay advances the drug discovery process, enabling high-throughput screening of compound libraries, identifying potential leads, and evaluating binding kinetics and affinities.
Lead Optimization: With the aid of our SPR binding assay, researchers can improve lead compounds by making structured changes and evaluating their binding kinetics.
Protein-protein Interactions: Investigate protein-protein interactions in a label-free format with our SPR assay, which provides an insight into protein complex formation, stability, and function, to develop targeted therapies.
Antibody Characterization: By employing the SPR binding assay for antibody characterization, you can evaluate the antibody-antigen interactions, assess binding affinities, selectivity, and epitope specificity.
Biomarker Detection and Diagnostics: Our SPR assay aids in identifying and quantifying biomarkers, develop sensitive and specific diagnostic assays for various diseases and conditions.
Enzyme Kinetics and Mechanisms: Study enzyme-substrate interactions that will provide information regarding enzymatic mechanisms, kinetics, and drug design opportunities.
Principles of SPR Binding Assay
Surface Plasmon Resonance binding assay is founded on the principle of detecting molecular interactions in real time, without the need for labels or tags. Our SPR assay techniques capitalizes on the distinct optical traits of a thin metal film, that is immobilized onto a glass substrate.
The fundamental concept at the core of Surface Plasmon Resonance is a phenomenon in which polarized light traverses the metal film it engages with free electrons at the metal’s surface. Culminating in the creation of a plasmon wave, causing a dip in reflected light intensity at a specific angle, known as the resonance angle, within the context of SPR binding assays, this process unfolds through stages.
Our SPR binding assay has the capability for label-free, real-time monitoring that provides invaluable data on binding kinetics, affinity, and specificity. This, in turn, will bolster your comprehension of molecular interactions, and underpins critical applications spanning drug exploration, biomarker identification, and foundational scientific examinations.
Enhancing Bioprocessing Efficiency with Biacore SPR Assay Services
SPR is the state of the art biophysical technique for characterizing biomolecular interactions, and our SPR assay services utilize the Biacore 8K/8K+ platform in order to provide high throughput fragment and small molecule screening (HTS). It also includes hit validation, clean screens, binding level screens, and titrations with full kinetics and affinity characterization – we cover it all:
- High-throughput clean screens & binding level screens
- Kinetic assays & titrations, single cycle (SCK), multicycle (MCK), parallel, and 2D
- Affinity assays & titrations: small molecule affinity determinations
- Thermodynamic studies, tease apart the thermodynamic parameters of your interaction
- Epitope binning, characterize and sort a library of monoclonal antibodies against a target protein
- Concentration & potency analysis: batch analysis of antibodies
Our Biacore Service: Enhancing Bioprocessing Efficiency with SPR Assay Precision
Precision Characterization: With its precision characterization of molecular interactions, you will be able to measure parameters like affinity, kinetics, and thermodynamics with our Biacore capabilities. Providing an understanding of molecular interactions, and paving the way towards development of effective bioprocessing protocols.
Streamlined Development: Expedite the development of biotherapeutics by identifying potential issues early in the process, allowing for swift adjustments, opportunities to save time and resources, and ensuring an efficient and successful bioprocess.
Robust Data: Our Biacore SPR binding assay support provides robust data output ensuring that your research is founded on reliable information, reducing the risk of experimental errors, and enhancing the overall credibility of your findings.
Custom Biacore Service and Collaborative SPR Assay Research
Our specialized Biacore service is designed to cater to your specific research requirements, our team of scientists will work closely with you to develop personalized assays, analyze data, and establish experimental parameters. Additionally, we actively seek collaboration with all of our clients. We work with you to ensure that your research is on the right track, and this collaborative approach ensures that all aspects of our Biacore support fits to your needs from beginning to end.