Routine tissue processing, embedding, sectioning, and staining – H&E, Picrosiruis Red, Masson’s Trichrome, PAS and other speciality stains.
Full library of validated markers for immunohistochemistry (IHC) and immunofluorescence (IF) staining. Optimization services offered for non-validated targets.
Blinded board-certified pathologists will evaluate whole slides and provide extensive pathology reports on tissue specimens.
Quantitative Image Analysis
In-house AI tools have the capability to quantify staining and signal intensity with both colorimetric and fluorescence workflows with custom masking analysis.
Physical slides can be converted into digitized slides for ease of access and high throughput sample chain of custody.
Unique Histology Capabilities
Ichor is always looking to expand our capabilities outside the standard scope of histology work. The following customizable specialty histology services are available.
Tissue Microarray Development
Spheroid Histology Services
Multi-Colored Immunofluorescence Confocal Microscopy
Tissue Specimen Quantification Example
In-house image analysis software turns digital slides into quantitative data packages! Our robust software quantification can target specific stains and various biomarkers from both healthy and diseased models. The above example displays our image quantification analysis on murine livers, quantifying phenotype-specific deposits found in diseased phenotypes. Our analysis, corroborated with board-certified pathology scoring, determined an age-dependent trend of large disease phenotypic deposits (>240µm).
Immunohistochemistry Staining Examples
Chromogenic IHC Staining
IHC utilizes chromogenic reagents to detect protein abundance, distribution, and localization. Chromogenic detection produces an insoluble colored product at the antigen site through enzymatic reaction. With multiple chromogenic IHC verified in-house techniques and validated antibodies we have the capability to produce customized protocols and antigen retrieval based on your project needs. Antigen signal intensity can be scored by board certified pathologists or customized image analysis of signal quantification
IF utilizes fluorophore conjugated antibodies to detect protein abundance, distribution, and localization. Our histology core has the capability to produce a four color staining panel with a nuclear marker. Confocal microscopy techniques are used to acquire representative images and confirm expected signal intensity. Antigen signal intensity can be scored by board certified pathologists or customized image analysis of signal quantification.