Bio-Layer Interferometry Services

The Octet RED384 has 16 channel throughput and the sensitivity to generate literature quality quantitation and kinetics data.

BLI Intro

Similar to surface plasmon resonance, BLI measures binding affinity and association/dissociation kinetics.

A variety of crosslinking chemistries are available for affixing one of the peptide or protein binding partners to the sensor tips. The sensor tips are then dipped into solutions of targets to detect and quantify binding.

The sensor tips are then dipped into solutions of targets to detect and quantify binding.

Association and dissociation data are collected, allowing for the time-resolved quantification of interactions and calculation of kinetic k_a and k_d constants.

BLI is an excellent technique for heavier analytes (>3-5 kDa) in complex matrices (e.g. serum samples) as there are no microfluidics.

Learn More About BLI:

Octet RED384 System Brochure

Label-free quantitation and kinetics with enhanced throughput and extended dynamic range.

Biosensor Selection Guide

There are many biosensors to choose from for both kinetics and quantitation, including Streptavidin, anti-HIS, & various antibody coatings.

Regeneration–Conditions & Considerations

Overview of biosensor regeneration and considerations for choosing the optimal biosensor.

Quantitation

Application note for a simple procedure to quantitate His-tagged protein using HIS1K biosensors.

Kinetics

Application note on how to run a simple kinetic analysis of His-tagged proteins using Ni-NTA biosensors.

BLI Publications:

We are Scientists & Innovators!

Kinetics of the multitasking high-affinity Win binding site of WDR5 in restricted and unrestricted conditions

Convergent Alterations of a Protein Hub Produce Divergent Effects within a Binding Site

Interplay of Affinity and Surface Tethering in Protein Recognition

BLI projects have fast turnaround times.

Our BLI projects start within 2 weeks of receipt of materials.