The Octet RED384 has 16 channel throughput and the sensitivity to generate literature quality quantitation and kinetics data.
Similar to surface plasmon resonance, BLI measures binding affinity and association/dissociation kinetics.
A variety of crosslinking chemistries are available for affixing one of the peptide or protein binding partners to the sensor tips. The sensor tips are then dipped into solutions of targets to detect and quantify binding.
The sensor tips are then dipped into solutions of targets to detect and quantify binding.
Association and dissociation data are collected, allowing for the time-resolved quantification of interactions and calculation of kinetic ka and kd constants.
BLI is an excellent technique for heavier analytes (>3-5 kDa) in complex matrices (e.g. serum samples) as there are no microfluidics.
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