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Protein Expression & Purification Services
Are you looking for protein purification and protein expression services?
Let us take care of construct design, expression testing, and purification scouting.
Our team has a decade of experience solving novel problems for our internal programs and for our clients. We have a vast majority of systems available for protein production from small to large scale along with standardized scouting methods to generate the most physiologically relevant protein for all your downstream needs!
Process of Protein Production
Protein production, including protein expression services and protein purification, is a biotechnical process of generating large quantities of a specific protein of interest using recombinant DNA technology. Protein production occurs through the transcription and translation of genetic information encoded in DNA, allowing for the use of these proteins in a variety of applications such as research, diagnostics, therapeutics, and industrial processes.
Protein Expression
We offer a wide range of protein expression services that utilize distinct expression systems, which are tailored to meet your specific project requirements. Our expertise extends to mammalian, insect, bacterial, and yeast expression systems, each offering unique advantages and capabilities.
Mammalian Expression
Utilizing HEK293 and CHO suspension cultures allows for the production of recombinant proteins and antibodies at large scale. Our optimized system, utilizing either transfection with plasmid DNA, transduction BacMam viruses produced in insect cells, or stable cell line development, allows for high-yielding and reproducible expression of intracellular, secreted and membrane proteins.
Insect Expression
Large scale production of intracellular, secreted, or membrane proteins using our baculovirus-mediated expression in insect cells. This allows for most of the post-translational modifications that occur in mammalian cells and results in high yields and fast turnaround times once baculovirus stocks have been generated.
Bacteria Expression
Using a variety of E. coli strains, our bacterial expression systems offer reliable and reproducible production of recombinant proteins at bioreactor scale, up to 40 L per batch.
Yeast Expression
Protein expression in yeast provides a system that can produce high yield and potential capacity for scale up of proteins that are hard to express in bacteria. The similarity of the secretory system in yeast to that in mammalian provides a means to produce proteins with near-native post-translational modifications. In addition, yeast cells can be genetic manipulated to produce functional versions of therapeutically important proteins.
Protein Purification
Protein purification refers to the isolation of a target protein from a complex mixture of other biological materials utilizing various bulk physicochemical properties such as size, charge and hydrophobicity. Isolating a protein to a purity of >90% is essential for their functional and structural characterization.
The process of protein purification consists of the following steps:
- Sample Preparation
- Protein Extraction
- Optimization and Purification
- Analysis and Quality Control
Not Sure Where To Start?
Our scientists bring decades of experience working with these expression systems along with the purification and characterization of both soluble and membrane proteins. From gene design and expression to purification scouting utilizing various buffers and chromatographic techniques, we are here to provide you with functional, sufficient protein to feed your downstream workflows. In addition, we can also provide advice on downstream application compatibility and recommend functional assays for your protein and can provide consultation for any aspect of your protein pipeline.
Through our protein expression services we test a variety of constructs with different tags using different expression conditions and systems in parallel. This allows us to find the most optimal conditions for expression to move into purification.
Besides the standard QC (SDS-PAGE and anSEC on pre- and post- thaw material), we offer binding assays, activity assays, crystallography services, and numerous biophysical assays through our biophysics and structure services that work in conjunction with our protein expression services.
Our in-house purification scouting method is designed to generate optimal conditions for your downstream experiments with the greatest yield and highest purity. We run parallel column scouting on any soluble or refolding prep to give the best information to develop a high-yielding, pure, and applicable purification cascade to facilitate any downstream needs.
Not sure which storage buffer to use? We can run formulation and lyophilization trials to get you the required formulation suitable for your downstream assays.
Frequently Asked Questions
Have a question that isn’t answered here? Reach out to our team of scientists and we will answer any questions you might have about our protein expression services!
With our protein expression services the completion of expression and purification scouting along with purification optimization is accomplished within 2 weeks of receiving genes. Scale up depends on the amount of material needed but once a purification cascade is established, productions can be initiated right away.
Upon initiation, our team of experts will review all the conditions with your team. All relevant information such as tags, cleavage sites, buffers, concentrations, yields, etc will be reviewed so we provide exactly what you are looking for.
We can use any information or methods you provide to express and purify protein according to what you would like. Our expression and purification scouting methods are designed to give the highest yielding, most relevant purification schemes but can be passed over based on your own desires.
Yes! We can always add a cleavage site to any construct for tag removal and have standardized procedures for cleavage and endotoxin removal to facilitate your needs.