Protein Expression & Purification Services
Looking for protein expression and purification services?
Let us take care of construct design, expression testing, and purification scouting.
Our team has a decade of experience solving novel problems for our internal programs and for our clients. We have a vast majority of systems available for protein production from small to large scale along with standardized scouting methods to generate the most physiologically relevant protein for all your downstream needs!
Mammalian Expression
Utilizing Expi293f and ExpiCHO-S suspension cultures allows for the production of recombinant proteins and antibodies at large scale. Our optimized system allows for reproducibility of intracellular, secreted and membrane proteins.
Insect Expression
Large scale production of intercellular, secreted, or membrane proteins using our baculovirus expression systems allows for most of the post-translational modifications of the mammalian expression with higher yields and quicker turnaround times after baculovirus stocks are generated.
Bacteria Expression
Using a variety of E. coli strains, our bacterial expression systems offer reliable and reproducible production and purification of recombinant proteins at bioreactor scale, up to 40 L per batch.
Yeast Expression
High product yield and scale up capacity, yeast offers easy genetic manipulation to produce proteins with desired properties unable to attain through bacterial cells.
Not Sure Where To Start?
Our scientists bring decades of experience and a diverse background to solving your problems. We can consult you on scouting conditions, troubleshooting, downstream application compatibility, and recommend functional assays for your protein.
We test a variety of constructs with different tags using different expression conditions and systems in parallel. This allows us to find the most optimal conditions for expression to move into purification.
Besides the standard QC (SDS-PAGE and anSEC on pre- and post- thaw material), we offer binding assays, activity assays, crystallography services, and numerous biophysical assays through our biophysics and structure services.
Our in-house purification scouting method is designed to generate optimal conditions for your downstream experiments with the greatest yield and highest purity. We run parallel column scouting on any soluble or refolding prep to give the best information to develop a high-yielding, pure, and applicable purification cascade to facilitate any downstream needs.
Not sure which storage buffer to use? We can run formulation and lyophilization trials to get you the required formulation suitable for your downstream assays.
Frequently Asked Questions
Have a question that isn’t answered here? Reach out to our team of scientists and we will answer any questions you might have!
Completion of expression and purification scouting along with purification optimization is accomplished within 2 weeks of receiving genes. Scale up depends on the amount of material needed but once a purification cascade is established, productions can be initiated right away.
Upon initiation, our team of experts will review all the conditions with your team. All relevant information such as tags, cleavage sites, buffers, concentrations, yields, etc will be reviewed so we provide exactly what you are looking for.
Ichor can use any information or methods you provide to express and purify protein according to what you would like. Our expression and purification scouting methods are designed to give the highest yielding, most relevant purification schemes but can be passed over based on your own desires.
Yes! We can always add a cleavage site to any construct for tag removal and have standardized procedures for cleavage and endotoxin removal to facilitate your needs.