Nuclear Magnetic Resonance (NMR)
NMR Comparability Studies for Biosimilars: Ichor has priority access to NMR services, the facilities, and high field instrumentation necessary to collect your FDA required biosimilarity data. With project management and bolt-on consulting and data analysis services, Ichor offers fast turnaround times to make your deadlines, in addition to personalized, attentive, and thoughtful collaboration.
What is NMR Spectroscopy?
Nuclear Magnetic Resonance (NMR) spectroscopy is a technique used to study the structure, composition, and dynamics of molecules, based on the principles of NMR, a phenomenon that is exhibited by certain atomic nuclei, when placed in a magnetic field, and exposed to radiofrequency radiation.
NMR spectroscopy involves the placement of a sample containing nuclei with magnetic properties, most commonly hydrogen-1, carbon-13, and others, into a strong magnetic field. Nuclei align with the magnetic field, a radiofrequency pulse is applied, disrupting the alignment, once the radiofrequency pulse is turned off, the nuclei return to their original alignment.
NMR spectroscopy encompasses a wide range of applications, some key aspects of NMR services include:
- Protein Structure Determination: Determine the three-dimensional structures of proteins, providing an insight into protein function and their interactions with other molecules.
- Protein Dynamics: Study the dynamic movements of proteins and understand how their flexibility relates to their biological function with our NMR services.
- Ligand Interaction Studies: NMR services investigate the interactions between proteins or nucleic acids and ligands, aiding in drug discovery, and development.
- Drug-Target Interactions: NMR spectroscopy can provide crucial information on how drugs interact with their target molecules, which will aid the design on optimization of new drugs.
Our NMR Services Capabilities:
High-Throughput FBDD and Binding Studies: Our NMR services include 1H and 19F binding assays (FAXS, STD-NMR, WaterLOGSY), in addition to various 2D correlation experiments such as 1H-1H (COSY, NOESY, ROESY), 1H-15N (SOFAST-HMQC) and 1H-13C (ALSOFAST-HMQC) correlation spectroscopy.
Multiple instruments are equipped with SampleJet robots, allowing for low volume and/or 96 well plate screening formats. Also offered are services for production of isotopically enriched recombinant proteins.
Popular FBDD and Biosimilar NMR Services
Ichor offers all the cutting-edge NMR experiments for Fragment-Based Drug Discovery and Biosimilar Comparability.
WaterLOGSY
A sensitive ligand-observed NMR experiment for detection of interactions between a ligand and a protein; well-established screening technique for FBDD
Data Acquisition
Ichor offers various 1D and 2D NMR experiments from everything from small molecules to antibodies and other complex biologics:
Simple 1D experiments are available for everything from small molecule and protein QC to HTS screening (FAXS, STD-NMR, waterLOGSY).
Two dimensional 1H-15N experiments are commonly used for both protein QC (fingerprinting coupled with PCA or Mahalanobis Distance calculations), as well as various protein-protein and protein-ligand binding experiments & titrations.
Available Spectrometers (priority access):
- 500 MHz (1)
- 600 MHz (2)
- 800 MHz (1)
All equipped with triple resonance TCI CryoProbes (1H,13C,15N) for fast, high-quality data collection.
Various multidimensional correlation experiments are also available. Custom pulse sequences can be developed upon request.
Two dimensional 1H-13C experiments are another commonly used technique for fingerprinting and QCing large biomolecules, including antibodies. Ichor offers isotopically enriched recombinant protein expression (2H, 13C, & 15N).
Options to facilitate screening:
- SampleJet robots (automation)
- 96-well plate format
- Low volume (65 µL)
- Temperature control
Add-On Services to Supply Your Entire Pipeline:
Consulting & Data Analysis
For more complex, nuanced, or data intensive projects, NMR specialists are available for hourly consultation.
Production of Isotopically Enriched Recombinant Proteins
We offer recombinant protein production with heavy isotope incorporation (2H, 13C, & 15N). Please inquire about discounts for bundling in-house protein production services with our analytical offerings.
