Nuclear Magnetic

High-Throughput FBDD and Binding Studies: Our services include 1H and 19F binding assays (FAXS, STD-NMR, WaterLOGSY), in addition to various 2D correlation experiments such as 1H-1H (COSY, NOESY, ROESY), 1H-15N (SOFAST-HMQC) and 1H-13C (ALSOFAST-HMQC) correlation spectroscopy.

Multiple instruments are equipped with SampleJet robots, allowing for low volume and/or 96 well plate screening formats. Also offered are services for production of isotopically enriched recombinant proteins.

Popular FBDD and Biosimilar NMR Services

Ichor offers all the cutting edge NMR experiments for Fragment-Based Drug Discovery and Biosimilar Comparability.


FBDD via 19F NMR–Fluorine chemical shift Anisotropy and eXchange for Screening experiments (FAXS).


Fragment Screening via STD-NMR –May also be used for Ligand Mapping and KD determination.


A sensitive ligand-observed NMR experiment for detection of interactions between a ligand and a protein; well-established screening technique for FBDD.

Distance Calculations

Using Mahalanobis Distance and PCE Analysis (1D & 2D) in the Comparison of Biosimilars.

2D Correlation Spectroscopy–Biosimilars

Application of 2D NMR “Fingerprint” Spectra to Compare Biosimilar mAb Therapeutics.

2D Correlation Spectroscopy–Binding Studies

Quantitate protein/ligand binding interactions via N-H Chemical Shift Perturbations (CSPs). Proteins <30 kDa are amenable to the technique.

Data Acquisition

Ichor offers various 1D and 2D NMR experiments from everything from small molecules to antibodies and other complex biologics:

Simple 1D experiments are available for everything from small molecule and protein QC to HTS screening (FAXS, STD-NMR, waterLOGSY).

Two dimensional 1H-15N experiments are commonly used for both protein QC (fingerprinting coupled with PCA or Mahalanobis Distance calculations), as well as various protein-protein and protein-ligand binding experiments & titrations.

Available Spectrometers (priority access):

  • 500 MHz (1)
  • 600 MHz (2)
  • 800 MHz (1)

All equipped with triple resonance TCI CryoProbes (1H,13C,15N) for fast, high-quality data collection

Various multidimensional correlation experiments are also available. Custom pulse sequences can be developed upon request.

Two dimensional 1H-13C experiments are another commonly used technique for fingerprinting and QCing large biomolecules, including antibodies. Ichor offers isotopically enriched recombinant protein expression (2H, 13C, & 15N).

Options to facilitate screening:

  • SampleJet robots (automation)
  • 96-well plate format
  • Low volume (65 µL)
  • Temperature control

Add-On Services to Supply Your Entire Pipeline:

STD-Difference Spectra

Consulting & Data Analysis

For more complex, nuanced, or data intensive projects, NMR specialists are available for hourly consultation.

2D NMR Ligand Titration image

Production of Isotopically Enriched Recombinant Proteins

We offer recombinant protein production with heavy isotope incorporation (2H, 13C, & 15N). Please inquire about discounts for bundling in-house protein production services with our analytical offerings.

Sample1_vs_Sample1 (1)
Sample1_vs_Sample2 (2)

Our NMR projects have fast turnaround times.

NMR studies start within 2 weeks of receipt of materials.