Isothermal Titration Calorimetry & Differential Scanning Calorimetry

Truly label-free, solution-based affinity determinations via Isothermal Titration Calorimetry (ITC) – TA Instruments Nano ITC.

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Protein Services Biophysical Assays Isothermal Titration Calorimetry (ITC)

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Using differential scanning calorimetry (DSC) - TA Instruments Nano DCS

  • Protein stability
  • Quality-control
  • Thermodynamics studies

What is ITC & DSC

ITC–Overview

ITC is a physical technique used to determine the thermodynamic parameters (KD, ΔH, ΔS, ΔG, stoichiometry) of binding partners in solution. Low nM – µM.

DSC–Overview

DSC, a close relative of ITC, is a thermoanalytical technique frequently used to measure protein structure & folding, as well as stability (e.g. ± ligands; mutations).

ITC Training Guide

Primer on microcalorimetry via ITC; reviews the theory, experimental design, and tips & tricks.

Competitive Binding Experiments

Overview of the design and execution of competitive inhibitor experiments via ITC, with particular relevance to fragment screening.

Obtaining Enzyme Kinetics with ITC

Application Note for using ITC to obtain enzyme kinetic constants.

Theory & Applications of the Nano-DSC

Primer on Nano DSC, applications, sample prep, data analysis, & case studies.

Related Products & Services:

ITC is an excellent secondary or orthogonal screen for hit validation and characterization, but a technique such as SPR is recommended for primary and high-throughput screening.

Biacore 8K & 8K+ (5)

High-Throughput Primary Screening via SPR

SPR_Plate-Compartment

SPR Services for High-Throughput Fragment Screening & Hit Validation

Fragment clean screens, binding level screens, & titrations for binding confirmation and kinetics.

Isothermal Titration

Most ITC & DSC studies start within 2 weeks of receipt of materials.

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