Plate-Based Assay Services
Ichor offers numerous plate-based assay services, including ELISA, Fluorescence Polarization (FP), & Time-Resolved Fluorescence Energy Transfer (TR-FRET).
Ichor also offers thermal shift assays (i.e. Differential Scanning Fluorimetry; DSF), useful for conducting protein quality control, buffer stability screens, and high-throughput compound screening.
Plate-Based Assay Services
Turnkey assay development and screening
ELISA
Enzyme-Linked Immunosorbent Assay (ELISA) is a commonly used analytical biochemistry assay. The technique is often used for detection and quantification of a desired analyte (e.g. protein/antigen) in solution. Kits are often commercially available, but Ichor can also develop assays de novo. ELISA is very sensitive, with a functional range of pg/mL–ng/mL.
Fluorescence Polarization (FP)
Fluorescence Polarization (FP) is a homogeneous, steady-state, solution-based assay involving a single fluorescently labeled binding partner that is used for the determination of the thermodynamic dissociation constant (KD). The technique is highly amenable to plate-based high-throughput library screening and hit discovery campaigns. Functional range: 1 nM to 1 mM.
Time-Resolved Fluorescence Energy Transfer (TR-FRET)
Time-Resolved Fluorescence Energy Transfer (TR-FRET) is a highly sensitive and robust drug discovery assay/technique involving fluorescently labeled donor and acceptor proteins that is used to determine both thermodynamic and kinetic parameters (KD, EC50, ka, kd). Functional range: 1 pM to 1 mM.
Thermal Shift Assays / Differential Scanning Fluorimetry (DSF)
Protein thermal shift assays are a technique used to measure the melting point (Tm) of a protein. Protein melting is useful for identifying ligands, buffer conditions, and mutations that maximize the stability of proteins during purification, crystallization, and functional characterization. The technique lends itself to high-throughput ligand screening. Requires <1 µg of sample per well.
Instrumentation:
We have the wet lab space, state-of-the-art instrumentation, and trained personnel to execute your projects
Molecular Devices SpectraMax i3x
We offer assay development on two molecular devices SpectraMax i3x temperature-controlled plate readers that can handle 96- and 384-well plates in absorbance, fluorescence, or chemiluminescent read modes.
- One i3 module is equipped with an automated StakMax plate hopper for automated library screening campaigns.
- One i3 module is equipped with a MiniMax 300 Imaging Cytometer
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