qPCR

Ichor also offers thermal shift assays (i.e. Differential Scanning Fluorimetry; DSF), useful for conducting protein quality control, buffer stability screens, and high-throughput compound screening.

Plate-Based Assay Services

Turnkey assay development and screening

ELISA-plate

ELISA

Enzyme-Linked Immunosorbent Assay (ELISA) is a commonly used analytical biochemistry assay. The technique is often used for detection and quantification of a desired analyte (e.g. protein/antigen) in solution. Kits are often commercially available, but Ichor can also develop assays de novo. ELISA is very sensitive, with a functional range of pg/mL–ng/mL.

Fluorescence-Polarization-Titrations

Fluorescence Polarization (FP)

Fluorescence Polarization (FP) is a homogeneous, steady-state, solution-based assay involving a single fluorescently labeled binding partner that is used for the determination of the thermodynamic dissociation constant (KD). The technique is highly amenable to plate-based high-throughput library screening and hit discovery campaigns. Functional range: 1 nM to 1 mM.

Establishing-Novel-FRET-image

Time-Resolved Fluorescence Energy Transfer (TR-FRET)

Time-Resolved Fluorescence Energy Transfer (TR-FRET) is a highly sensitive and robust drug discovery assay/technique involving fluorescently labeled donor and acceptor proteins that is used to determine both thermodynamic and kinetic parameters (KD, EC50, ka, kd). Functional range: 1 pM to 1 mM.

Thermal Melt Curve

Thermal Shift Assays / Differential Scanning Fluorimetry (DSF)

Protein thermal shift assays are a technique used to measure the melting point (Tm) of a protein. Protein melting is useful for identifying ligands, buffer conditions, and mutations that maximize the stability of proteins during purification, crystallization, and functional characterization. The technique lends itself to high-throughput ligand screening. Requires <1 µg of sample per well.

Instrumentation:

We have the wet lab space, state-of-the-art instrumentation, and trained personnel to execute your projects

Molecular Devices SpectraMax i3x

We offer assay development on two molecular devices SpectraMax i3x temperature-controlled plate readers that can handle 96- and 384-well plates in absorbance, fluorescence, or chemiluminescent read modes.

  • One i3 module is equipped with an automated StakMax plate hopper for automated library screening campaigns.
  • One i3 module is equipped with a MiniMax 300 Imaging Cytometer

Thermo QuantStudio 3 qPCR

We offer assay development on a Thermo QuantStudio 3 qPCR capable of performing thermal melt assays.

Publications:

We are scientists & innovators!

Fluorescence-Polarization-Titrations

Kinetics of the multitasking high-affinity Win binding site of WDR5 in restricted and unrestricted conditions

pub4

RPtag as an Orally Bioavailable, Hyperstable Epitope Tag and Generalizable Protein Binding Scaffold

Fluorescence-Polarization-Detergents

High-Throughput Screening of Protein-Detergent Complexes Using Fluorescence Polarization Spectroscopy

  • Development & deployment of new protein-based tools
  • Problem agnostic approaches
  • Solution-based high-throughput screening & affinity determination
  • Capability to run 50 × 384 well plates per day!

Testimonials

Client satisfaction is our measure of success

“Ichor helped us develop a custom binding assay that was critical for selection and optimization of our lead molecule. Throughout the process, the Ichor team was highly collaborative and flexible and provided high quality data in a timely manner”

– Ankyra Therapeutics

Want more information?

Schedule a meeting with our scientists.