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Plate-Based Assay Services
Ichor offers numerous plate-based assay services, including ELISA, Fluorescence Polarization (FP), & Thermal Shift Assays.
Ichor offers a variety of kit-based or custom plate-based assay services—pretty much any assay that can be run on a standard 96- or 384-well plate in absorbance, fluorescence, or chemiluminescent read modes.
Plate-Based Assay Services
Turnkey assay development and screening
ELISA
Enzyme-Linked Immunosorbent Assay (ELISA) is a commonly used analytical biochemistry assay. The technique is often used for detection and quantification of a desired analyte (e.g. protein/antigen) in biological samples. Kits are often commercially available, but Ichor can also develop assays de novo. ELISA is very sensitive, with a functional range of pg/mL–ng/mL.
Fluorescence Polarization (FP)
Fluorescence Polarization (FP) is a homogeneous, steady-state, solution-based assay involving a single fluorescently labeled binding partner that is used for the determination of the thermodynamic dissociation constant (KD). The technique is highly amenable to plate-based high-throughput library screening and hit discovery campaigns. Functional range: 1 nM to 1 mM.
Time-Resolved Fluorescence Energy Transfer (TR-FRET)
Time-Resolved Fluorescence Energy Transfer (TR-FRET) is a highly sensitive and robust drug discovery assay involving fluorescently labeled donor and acceptor proteins that is used to determine both thermodynamic and kinetic parameters. Functional range: 1 pM to 1 mM.
Thermal Shift Assays / Differential Scanning Fluorimetry (DSF)
Protein thermal shift assays are a technique used to measure the melting point (Tm) of a protein, which is the temperature at which there is 50% denaturation. Thermal shift assays are useful for evaluating protein quality and buffer stability, as well as identifying (de)stabilizing mutations or small molecule binders. The technique also lends itself to high-throughput ligand screening. Requires <1 µg of sample per well.
Instrumentation:
We have the wet lab space, state-of-the-art instrumentation, and trained personnel to execute your projects
Molecular Devices SpectraMax i3x
We offer assay development on two molecular devices SpectraMax i3x temperature-controlled plate readers that can handle 96- and 384-well plates in absorbance, fluorescence, or chemiluminescent read modes.
- One i3 module is equipped with an automated StakMax plate hopper for automated library screening campaigns.
- A separate i3 module is equipped with a MiniMax 300 Imaging Cytometer
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