Human General Phenotyping 26c

Cell populations and gating definitions.

Population Gating Definition
Basophils CD45+CD123+HLA-DR-CD11c-
Monocytes
Classical MonocytesCD45+SSCHiCD14+CD16-
Intermediate MonocytesCD45+SSCHiCD14+CD16+
Non-Classical MonocytesCD45+SSCHiCD14-CD16+
Lymphocytes
LymphocytesCD45+SSClo
ILCsCD45+(Lin-)CD3-TCRγδ-CD19-CD20-CD14-CD123-CD16-HLA-DR-CD4-CD8-CD127+
Dendritic Cells
pDCsCD45+CD3-TCRγδ-CD19-CD56-CD14-HLA-DR+CD123+CD11c-
DCsCD45+CD3-TCRγδ-CD19-CD56-CD14-HLA-DR+CD123-CD11c+CD16+
CD141+ DCCD45+CD3-TCRγδ-CD19-CD56-CD14-HLA-DR+CD123-CD11c+CD16-CD141+
CD1c+ DCCD45+CD3-TCRγδ-CD19-CD56-CD14-HLA-DR+CD123-CD11c+CD16-CD141-CD1c+
B Cells
Naïve B CellCD45+CD3-TCRγδ-CD19+CD20+IgD+CD27-
Memory B CellsCD45+CD3-TCRγδ-CD19+CD20+IgD+CD27+
Memory B Cells Class SwitchedCD45+CD3-TCRγδ-CD19+CD20+IgD-CD27+
PlasmablastCD45+CD3-TCRγδ-CD19+CD20+CD27+CD38HI
NK / NKT
High Cytotoxic NK cellsCD45+CD3-TCRγδ-HLA-Dr- CD56dimCD16+
Cytokine producing NK cellsCD45+CD3-TCRγδ-HLA-Dr- CD56brightCD16lo
NKT cellsCD45+CD3-TCRγδ-CD56+
T Cells
Gamma Delta T cellsCD45+CD3+TCRγδ+CCR7 vs CD45RA
Helper T Cells (CD4)
Helper T CellsCD45+CD3+TCRγδ-CD4+CD8-
Naïve CD4+ Helper T cellsCD45+CD3+TCRγδ-CD4+CD8-CCR7+CD45RA+
Central Memory CD4+ Helper T cellsCD45+CD3+TCRγδ-CD4+CD8-CCR7+CD45RA-
Effector CD4+ Helper T cellsCD45+CD3+TCRγδ-CD4+CD8-CCR7-CD45RA-
Effector Memory CD4+ Helper T cellsCD45+CD3+TCRγδ-CD4+CD8-CCR7-CD45RA-CD28vxCD27
Regulatory T cells (Tregs)CD45+CD3+TCRγδ-CD4+CD8-CD127-CD25+CCR7 vs CD45RA
Cytotoxic T Lymphocytes (CD8)
Cytotoxic T LymphocytesCD45+CD3+TCRγδ-CD4-CD8+
Naïve Cytotoxic T cellsCD45+CD3+TCRγδ-CD4-CD8+CCR7+CD45RA+
Central Memory Cytotoxic T cellsCD45+CD3+TCRγδ-CD4-CD8+CCR7+CD45RA-
Effector CTLCD45+CD3+TCRγδ-CD4-CD8+CCR7-CD45RA-
Effector Memory CTL (TEMRA)CD45+CD3+TCRγδ-CD4-CD8+CCR7-CD45RA+
Human General Phenotyping panel

The Human General Phenotyping (26-Color) Panel is a high-parameter, pre-optimized flow cytometry panel designed to deeply characterize major immune cell subsets in human peripheral blood mononuclear cells (PBMCs). It enables robust identification of innate and adaptive immune populations in a single staining tube, reducing the time and effort required for complex panel design

Key Features & Benefits

Efficiency

26-color, pre-optimized configuration reduces panel development time.

Broad Immune Coverage

T cells, B cells, NK cells, monocytes, dendritic cells, ILCs, basophils.

Optimization

Optimized antibody–fluorochrome pairing minimizes spectral overlap.

Superior Gating

Validated for human PBMCs with clean, reproducible gating.

Workflow

Streamlined workflows allow researchers to focus on data interpretation.

Target Immune Cell Populations

The panel resolves major immune lineages including T cells (CD4+, CD8+, memory, Tregs), B cell subsets (naïve, memory, plasmablasts), NK cell subsets (CD56bright/dim), ILC populations, monocyte subsets, dendritic cells, and basophils.

Featured Case Studies / Capabilities

T and NK cells

Human peripheral blood mononuclear cells were stained with the Human General Phenotyping (26c) panel. γ/δ T cells are depicted as TCRγ/δ+ CD3+ cells (A, top right). Multiple NK populations were identified as CD3- TCRγ/δ+ HLA-DR- triple-negative cells (B, bottom left). α/β T cells, defined as CD3+ TCRγ/δ- CD56- cells, are further divided into CD4+ and CD8+ T cell populations (C, bottom right). All data were gated on CD45+ viable single cells within the lymphocyte population.

CD4+ and CD8+ T cells


Human peripheral blood mononuclear cells were stained with the Human General Phenotyping (26c) panel. CD4+ T cell subsets, including CD25+ CD127- Tregs (A, bottom left) and CD45RA- CCR7- effector memory T cells (TEM) (B, bottom middle) are shown. CD8+ T cell subsets, including CD45RA- CCR7- effector memory T cells (TEM) (C, bottom right) are also shown. All data were gated on CD45+ CD3+ TCRγ/δ- CD56- viable single cells within the lymphocyte population.

B Cells

Human peripheral blood mononuclear cells were stained with the Human General Phenotyping (26c) panel. CD27+ IgD- memory B cells (A, top right) were further distinguished as CD20- plasmablasts (B, bottom left) and CD20- memory B cells (C, bottom right). All data were gated on CD45+ CD19+ CD3- TCRγ/δ- viable single cells within the lymphocyte population.

Dendritic cells and monocytes


Human peripheral blood mononuclear cells were stained with the Human General Phenotyping (26c) panel. Dendritic cells (DCs) were identified as CD45+ HLA-DR+ TCRγ/δ- CD3- CD56- CD19-- cells in the lymphocyte gate (top left). Classical DC subsets are further analyzed (top right and bottom right). Non-classical (CD14-CD16+), intermediate (CD14⁺CD16⁺), and classical (CD14+CD16-) monocytes are shown as viable CD45+ single cells within the monocyte population (bottom left).

Basophils


Human peripheral blood mononuclear cells were stained with the Human General Phenotyping (26c) panel. CD45int CD123+ basophils (A) were gated on total viable single cells.

Innate Lymphoid Cells

Human peripheral blood mononuclear cells were stained with the Human General Phenotyping (26c) panel. Data shown are gated on CD45+ CD3- TCRγ/δ- CD19- CD20- CD14- CD123- CD16- HLA-DR- cells in the lymphocyte population.

Recommended Application

The Human General Phenotyping (26-Color) Panel is designed for researchers who are in search of a comprehensive method for understanding the immune system. With a single staining tube, researchers can capture the full picture of innate and adaptive immunity, making this panel an ideal solution for your next program.

In immuno-oncology, the panel helps teams quickly identify key changes in T cells, NK cells, monocytes, and dendritic cells—critical indicators of how patients are responding to checkpoint inhibitors, CAR-T therapies, or other immune-modulating treatments. With clear resolution of activation and memory states, researchers gain actionable insights that support biomarker discovery and patient-stratification strategies.

In autoimmune and inflammatory disorders, the panel delivers a balanced look at dysregulated immune pathways by simultaneously measuring T, B, and innate subsets. This makes it easier to pinpoint abnormal immune patterns, understand disease mechanisms, or assess patient responses to therapies such as biologics or targeted immune modulators.

The panel also excels in immune reconstitution monitoring following transplantation, stem-cell therapy, or cytotoxic treatments. It provides a clear understanding of how the immune system rebuilds—tracking the return of naïve and memory T cells, the re-establishment of B cell populations, and the normalization of innate cell numbers. These insights help researchers spot delayed or atypical recovery early and adjust care strategies accordingly.

Finally, the panel is ideal for large-scale cohort studies and baseline immune profiling. Because it covers so many immune subsets at once, it offers a streamlined way to compare immune signatures across populations, identify trends, and build immune reference maps for systems-level research.

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