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Quantitative Receptor Internalization
Explore our receptor internalization studies to compare the level of a certain receptor on the surface of the cells and intracellularly after a certain time of incubation.
Ichor Life Sciences performs receptor internalization studies using flow cytometry for precise, quantitative measurement over time. This approach tracks receptors at both the cell surface and inside the cell, providing a full view of internalization kinetics.
Antibody-Drug Conjugates (ADC)
ADC target specific cell surface protein and deliver their target following internalization.
Therapeutic Antibodies
Therapeutic antibodies bind with high affinity to specific receptors or molecules on the surface of the cells and lead to internalization following binding.
Bivalent Antibodies
Many therapeutic antibodies contain multiple binding sites to facilitate receptor cross-linking at the cell surface which can trigger internalization.
Bispecific Antibodies (BsAb)
Bispecific antibodies target different receptors on the surface of cells and can lead to internalization.
Bispecific T cell engagers (BiTes)
Bispecific T cell engagers lead to cross-linking of T cells to cancer cells leading to TCR internalization and activation of T cell killing.
Featured Capabilities
Our flow cytometry research team utilizes industry-best equipment to conduct studies. Quality equipment for quality results.
Your Results Await
Cell expressing a receptor of interest are incubated with a novel monoclonal antibody targeting the protein. Binding is performed at 4 °C to prevent internalization. Then cells are transferred to 37°C to allow internalization. At designated time-points, cells are washed with ice cold PBS to stop internalization. The novel antibody is detected with a secondary antibody. Cells can also be fixed and permeabilized to detect the receptor antibody combination in the intracellular compartment. Samples left at 4°C for the same duration show much less intracellular staining. This novel monoclonal antibody triggers internalization of its receptor upon binding.