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Mammalian Expression System
The mammalian expression system provides the benefits of proper folding and post-translational modifications best suited for human or mammalian proteins. With over 10 years of experience in mammalian expression, Ichor offers transient to stable expression in HEK293 or CHO at large scale.
Our custom mammalian production services allow our clients the accessibility, flexibility and transparency that is highly sought after. With over 10 years of bacterial protein production, our experts will work with your team to provide the protein that will best suite your downstream needs
Gene Design
Genes are designed by our experts to fulfill your requirements.
- Timeline: 2-3 weeks
- Deliverables: Full sequence
DNA Amplification
Using our in-house DNA amplification services, we generate high quality and pure plasmid to support all downstream needs.
- Timeline: ≤ 1 week
- Deliverables: Full DNA report
Expression Testing
Small scale cultures to measure expression levels from transient transfection in either HEK293 or CHO suspension cells. One step capture of protein of interest from cells or culture supernatant.
- Timeline: 1-2 weeks depending on expression pathway
- Deliverables: Expression results (SDS-PAGE, A280)
Purification Optimization (Optional)
Utilizing an optimized system to evaluate the most pertinent purification cascade, our purification optimization allows us to establish pathways to achieve the highest yield and purity possible from mammalian expression.
- Timeline: ≤ 2 weeks
- Deliverables: Any available protein. Detailed protocol of work, Coomassie stained SDS-PAGE gels, chromatograms and analytical size exclusion if applicable.
Batch Productions
Our mammalian expression system allows for reliable and reproducible batch productions using the purification cascade established.
- Timeline: 1-2 weeks
- Deliverables:
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- All protein that is available.
- Analysis on Coomassie-blue stained SDS-PAGE gels.
- Analysis from analytical size-exclusion chromatography to probe for purity and monodispersity of preps if efficient yield is achieved.
- Full protein report for each preparation.
HEK293 & CHO Expression Platforms
HEK293 and CHO cells remain the gold standard for mammalian protein expression. HEK293 systems enable rapid, high‑level transient expression, while CHO platforms are ideal for stable expression and proteins requiring consistent human‑like glycosylation patterns.
Endpoints
Purity Assessment
Proteins are evaluated using SDS‑PAGE and chromatography to confirm purity and integrity.
Functional Readiness
Proteins are delivered suitable for biochemical, structural, or cell‑based assays.