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B16-F10 Mouse Model
Melanoma is the deadliest form of any skin cancer. Roughly half of all melanoma diagnosis are considered invasive, and incident level has been increasing significantly over the course of the last few decades.
B16-F10 is a well-documented immortal mouse melanoma cell line developed from the tenth passage of a B16 melanoma isolated from a C57BL/6 mouse.
Fast Growing Tumors
Our model reflects late-stage tumorigenesis, with mean tumor burden in experimental controls reaching euthanasia criteria within twenty-five days of inoculation.
Fully Characterized Model
This model can be used in orthotopic (flank) models for primary tumors or for lung metastases models when injected intravenously.
B16-F10 Melanoma Cells
B16 parental cells were originally isolated from a spontaneous tumor on the skin of a C57BL6 mouse well suited for metastatic disease studies.
Following isolation of the parental cells, passage in vivo led to the development of sub-cell lines with distinct metastatic properties. B16-F10 is highly metastatic and the cells exhibit both spindle-shaped and epithelial-like cell morphology in culture.
B16-F10 are suitable for use in both syngeneic and immunocompromised models. Depending on the route of administration, studies can be designed to analyze primary tumors and/or secondary lung metastases.
This model demonstrates proper tumor regression following standard of care in melanoma oncotherapy.