cnv

Choroidal neovascularization (CNV) is a sight-threatening condition associated with age-related macular degeneration (AMD). The CNV model has been developed to better comprehend this condition, and to aid in the development of effective treatment strategies. It provides a platform for studying disease mechanisms, testing potential therapies, and advance understanding of ocular vascular diseases.

Utilizing the advanced Phoenix MICRON® IV Laser guided system, our team is capable of accurate and repeated laser photocoagulation burns at the Bruch’s membrane. Additionally, we have enhanced drug delivery capabilities that allow us to directly compare targeted tissues and advance efficacy.

What is Choroidal Neovascularization (CNV)?

Choroidal neovascularization occurs when abnormal blood vessels grow from the layer behind the retina, called the choroid, into the retina. This abnormal vasculature can result in bleeding, fluid leakage, and scarring, leading to central vision impairment, common causes of CNV include age-related macular degeneration (AMD), myopia, and other retinal vascular diseases.

Laser-Induced CNV Model

The laser-induced choroidal neovascularization model is the most widely used form of CNV model in research, it is particularly favored for its ability to replicate the pathological processes seen in age-related macular degeneration and other CNV-related conditions. 

This model uses lasers that induce a thermal effect, promoting the growth of abnormal blood vessel from the choroid to the retina, leading to a rupture. By allowing for the precise control over the size and location of induced CNV, it provides conditions for consistent and reproducible experiments for different stages of CNV, disease progression, and will help in the research of potential interventions.

Immunohistochemistry

A method which utilizes antibodies to identify and locate particular proteins in ocular tissue sections, this technique proves useful in the identification and characterization of neovascular lesions, as it allows for the detection of specific proteins associated with CNV.

Electroretinography (ERG)

ERG evaluates the retina’s electrical activity in response to light stimulation, offering insights into retinal function and possible negative impacts of CNV induction or treatments on retinal well-being.

Applications of the Choroidal Neovascularization Model

  • Study of wet AMD (age-related macular degeneration)
  • Testing the Efficacy of Therapeutics
  • Drug Screening and Development
  • Mechanism of Action Studies
  • Evaluation of Gene Therapies
  • Assessment of Imaging Techniques
  • Evaluation of Combination Therapies

Types of CNV Model

Studying the pathogenesis and treatment options for choroidal neovascularization requires diverse models to replicate its conditions. These CNV models are used to investigate controlled angiogenic processes, cellular and molecular events, and test the efficacy of anti-angiogenic therapies.

Matrigel CNV Model

This choroidal neovascularization model imitates changes associated with the condition by injecting a gelatinous protein mixture into the subretinal space. It provides a method for studying controlled angiogenic processes, examining cellular and molecular events in angiogenesis, and testing anti-angiogenic therapies.

Transgenic Mouse Models

Transgenic Mouse CNV Models enable the modification of mouse genes to recreate the genetic mutations linked to choroidal neovascularization in humans. Providing a better understanding of the genetic basis of CNV and help in the development of targeted therapies.

Inflammatory Models

CNV pathogenesis is heavily influenced by inflammation, and inflammatory CNV model induces inflammation in the retina or choroid to provoke an immune response, leading to the growth of abnormal blood vessels. By assessing the impact of inflammation and testing anti-inflammatory treatments, researchers can understand CNV development and potential therapies.

Cell-Culture Models

Choroidal neovascularization processes can be stimulated using in vitro cell culture CNV models, in order to study cellular behaviors and potential treatments. Allowing for precise experimentation and drug testing, enabling early-stage drug discovery for choroidal neovascularization.

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Our CNV model will aid your research endeavours.

CNV Modeling

Endpoint Data Collection

Using the SD-OCT we capture retinal cross section scans for real time lesion volume measurements

In order to assess the area of leakage we use fluorescein angiography to measure both area and intensity

Onboarding results

CNV-Modeling-image

We take in-life weekly volumetric measurements at each lesion location

CNV Modeling

Our technique uses IV injections for fluorescein angiography to provide the highest quality angiograms and consistency in fluorescent intensities